Out of 12 fungal strains, two fungal strains, namely aspergillus flavus at2 and aspergillus niger at3, were found to produce maximum cellulase production. Article history abstract received 10 february, 2015 received in revised form 15 march, 2015. Optimization of cellulase production by aspergillus niger. Production of cellulase enzyme by mutant fungal strain aspergillus niger in submerged fermentation article pdf available in biotechnology. Cellulase production from species of fungi and bacteria from. Department of microbiology, faculty of science, university of abuja, abuja, nigeria.
To evaluate the potential of adaptive evolution as a tool in generating strains with an improved production of plant biomass degrading enzymes. Aspergillus niger, cellulase, carboxymethyl cellulose, mixed culture, trichoderma reesei introduction cellulase is a group of hydrolytic enzymes which abundant in nature kaur and joshi 2015. Sep 01, 2011 the results of smf process in the present study confirm the findings of devanathan et al and kathiresan and manivannan for aspergillus sp. These fungal species could be utilized in various industries. From this study, aspergillus niger and trichoderma viridae have high enzyme activity in pineapple peel. Mp biomedicals cellulase from aspergillus niger life. In order to compare the enzymes production, the determined optimum ssf conditions were used by t. Enhanced production of cellulase by escherichia coli. The results of smf process in the present study confirm the findings of devanathan et al and kathiresan and manivannan for aspergillus sp. An experimental approach for the production of cellulases by aspergillus niger from effluents of cotton ginning mill cellulases have enormous potential in industries and are used in food.
Microbial cellulases production, applications and challenges rajeev k sukumaran, reeta rani singhania and ashok pandey biotechnology division, regional research laboratory, industrial estate p o, trivandrum 695 019 microbial cellulases find applications in various industries and constitute a major group of the industrial enzymes. The phase of the cellulase enzyme production begins with the selection of microbes that being used which is trichoderma reesei with the consideration that the type of microbes capable of producing endo. Cellulase from aspergillus niger catalyzes the hydrolysis of endo1,4. Aspergillus niger is a species of great industrial importance besides koijmolds. Food and drug administration, it is widely used in the industrial production of citric acid and a variety of industrial enzymes such as amylases, pectinases, and proteases godfrey and west 1996. Optimization for cellulase production by aspergillus niger. Fungal cellulases are wellstudied enzymes and are used in various industrial processes.
Pdf cellulase production by wild strains of aspergillus. Deepesh 4 1department of biotechnology and molecular biology bioaxis dna research centre pvt. Different cultural conditions were examined to assess their effect in optimizing enzyme production. Pdf production of cellulase by aspergillus niger under submerged. Various physical and chemical conditions were employed in examining their influence on cellulase production by the selected fungal strain. Out of selected five fungal species aspergillus niger was observed as a rapidly cellulose degrading fungi because it gave the rapid and higher growth on the 1% cmc containing potato dextrose agar plate. Original research enhanced cellulase production from.
Pdf production of cellulase by aspergillus niger under. Production of cellulases by fungal cultures isolated from forest litter soil. Cellulase production from spent lignocellulose hydrolysates. Agro and kitchen wastes supported the growth and cellulase production in ssf.
It was the goal to investigate the cellulase enzyme production ability of fungal strains such as. Aspergillus niger was identified to be the best producer of cellulase. Production of cellulase by aspergillus niger under submerged and. Optimization for the production of cellulase enzyme from.
Much of the knowledge of enzymatic depolymerization of cellulosic material has come from trichoderma cellulase system. Pdf cellulase production from aspergillus niger ms82. Cellulase from aspergillus niger catalyzes hydrolysis of endo1,4. Trichoderma reseei, which is an established culture for cellulase production was used as a benchmark for the production of cellulase by a. Aspergillus niger and trichoderma viridae were screened for their cellulase production ability. Microscopic identification and morphology study revealed that the fungi isolated was aspergillus niger pectinase production media was later used for the lab scale production of pectinase enzyme by inoculating a. Optimization of cellulase production by aspergillus niger isolated. A recombinant aspergillus niger strain expressing the hypocrea jecorina endoglucanase cel7b was grown on spent hydrolysates stillage from sugarcane bagasse and spruce wood. Cellulase production by aspergillus unguis in solid state. Production of cellulase by aspergillus niger biofilms.
Prickly palm cactus husk was used as a solidstate fermentation support substrate for the production of cellulolytic enzymes using aspergillus niger and rhizopus. Research article enhanced production of cellulase on. Bagasse powder is used as a substrate for cellulase production employing novel thermostable yeast. The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. Pdf cellulase production by aspergillus niger on different natural. It was the goal to investigate the cellulase enzyme production ability. Physiological properties of this mutant revealed a five times higher cellulose production than the parental strain. Production and optimization of cellulase enzyme using. Cellulase production by aspergillus niger and saccharomyces. This problem can be overcome by the addition of extra bgl, e. Dglycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. Mar 17, 2018 this study examined cellulase production by a newly isolated aspergillus unguis on individual lignocellulosic substrates in solid state fermentation ssf. Sigmaaldrich offers a number of cellulase from aspergillus niger products.
Production of cellulases from aspergillus niger ns2 in solid. The fungal culture aspergillus niger used in the present study was isolated from the forest soil 18srilakshmi a, narasimha g. Pdf aspergillus niger was used for cellulase production in submerged smf and solid state fermentation ssf. An aspergillus niger cellulase mutant was obtained by adaptive evolution. Aspergillus niger was used for cellulase production in submerged smf and solid state fermentation ssf. In podospora anserina deletion of this gene panoxr resulted in reduced cellulase production brun et al. Deepesh 4 1department of biotechnology and molecular biology bioaxis dna research centre pvt ltd, lucknow, india. Improving cellulase production by aspergillus niger using. In the present investigation, we aim to isolate the two novel cellulase producing fungi aspergillus niger and trichoderma sp. Pdf optimization for cellulase production by aspergillus. The present study focused on a preliminary evaluation of cellulase enzyme production by aspergillus niger on.
Research article cellulase production by aspergillus niger. Asquieri and park found that the optimum temperature for production of cmcase from thermostable aspergillus sp. Original research enhanced cellulase production from isolated. Cellulase production was analysed in agroindustrial waste such as pineapple peel, orange peel and banana peel. Highlights we propose a low cost medium for production of cellulases by aspergillus niger. Biofilm and solidstate fermentations were carried out on perlite as inert support, and lactose was used as a carbon source in the three culture systems. Table 2 temperature vs cellulase production the cellulase production was optimized for rice bran using different temperature range of.
Optimization of cellulase enzyme production from trichoderma. Production of cellulase from aspergillus niger and. Pdf cellulases are the group of hydrolytic enzymes filter paperase fpase, carboxymethylcellulase cmcase and glucosidase bgl and. Aspergillus niger an overview sciencedirect topics. Production of cellulases from aspergillus niger ns2 in. The use of ssf cultivation under controlled conditions substantially improved cellulase production. The cmcase and fpase activities recorded in ssf were 8. The study reveals that aspergillus niger has the capability of producing cellulase and pectinase using corn cobs under ssf 20. Cellulase production by aspergillus niger was compared in three different culture systems. Production of cellulose by aspergillus niger under. Food and drug administration, it is widely used in the industrial production of citric acid and a variety of industrial enzymes such as. Production of cellulase by aspergillus niger under submerged and solid state fermentation using coir waste as a substrate. This study examined cellulase production by a newly isolated aspergillus unguis on individual lignocellulosic substrates in solid state fermentation ssf. Production of cellulase by different coculture of aspergillus niger and penicillium chrysogenum from waste paper, cotton waste and baggase m.
Production of cellulase by aspergillus niger under submerged. Successful utilization of cellulosic materials as renewable carbon sources is dependent on the development of economically feasible process technologies for cellulase production, and for the enzymatic hydrolysis of cellulosic materials to low molecular weight products such as hexoses and pentoses. Improving cellulase production by aspergillus niger using adaptive. Egypt accepted for publication, march 4, 1982 abstract cellulase enzyme was produced by a selected strain of aspergillus niger isolated from deteriorated wood and grown on different carbon sources. Successful utilization of cellulosic materials as renewable carbon sources is dependent on the development of economically feasible process technologies for cellulase production, and for the enzymatic hydrolysis of cellulosic materials to low molecular weight products such. The maximum production of cellulase was obtained after 72 h of incubation in ssf and 96 h in smf. Kitchen residues were better suited for inducing complete cellulase system. Cellulase enzyme produced in this study is the enzyme cellulase from fungi trichoderma reesei and aspergillus niger rice straw as the substrate. Thus, we hypothesized a similar role in the regulation of cellulase production in a. Optimization for cellulase production by aspergillus niger using paddy straw as substrate.
Comparative study of cellulase production by aspergillus. Microbial cellulases production, applications and challenges. Optimization for cellulase production by aspergillus niger using saw. Study on the use of agricultural wastes for cellulase. Throughout this study, the fungal strain aspergillus niger was maintained under the submerged condition for a period of 7 days at 120 rpm rotational speed. Appreciable levels of cellulases were produced over a wider environmental conditions. The maximum peak production of enzymes varied from one substrate to another, however, based on the next best solid support and local availability of groundnut fodder supported maximum enzyme. Cellulase production by the fungi aspergillus niger and saccharomyces cerevisiae was studied using pineapple and orange peels fruit wastes as substrates. Brazilian journal of microbiology,2011, 42, 11191127.
Optimization of cellulase production by aspergillus niger isolated from forest soil. Pdf cellulase production by aspergillus niger in biofilm. Production of cellulase by aspergillus niger under submerged and solid state fermentation using coir waste as a substrate article pdf available in brazilian journal of microbiology 423. Incubation period on cellulase production was determined. The spent hydrolysates served as excellent growth media for the cel7bproducing strain, a.
Chinedu and others published cellulase production by wild strains of aspergillus niger, penicillium chrysogenum and trichoderma harzianum grown on waste cellulosic. Production of cellulose by aspergillus niger under submerged. A good method for management of agro and kitchen waste residues. The organisms were screened for the production of the pectinase enzyme using pectin agar media. These agro industrial residues are cheap raw materials for cellulase production. Cellulase production from spent lignocellulose hydrolysates by recombinant aspergillus niger bjo. Regulation and improvement of cellulase production. In untreated biomass enzyme production by fungi was quite low thus emphasizing the need of pretreatment for biodegradation of forest lignocellulosics. The addition of this cellulase to lignocellulosic biomass showed good degradation of cellulose and therefore this fungus could be a future source for production of bioethanol from lignocellulosic biomass lb.
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